› 7xjk

Electron Microscopy
3.3Å resolution

Cryo-EM structure of the galanin-bound GALR2-miniGq complex

Released:
DOI: 10.2210/pdb7xjk/pdb
PDB entry 7xjk coloured by chain, front view.
PDB entry 7xjk coloured by chain, side view.
PDB entry 7xjk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into galanin receptor signaling.
Jiang W, Zheng S
OpenAccess logo Proc Natl Acad Sci U S A 119 e2121465119 (2022)
PMID: 35594396
Related structures: EMD-33230

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-208085 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Galanin Chain: A
Molecule details ›
Chain: A
Length: 30 amino acids
Theoretical weight: 3.16 KDa
Source organism: Homo sapiens
Expression system: synthetic construct
UniProt:
  • Canonical: P22466 (Residues: 33-62; Coverage: 29%)
Gene names: GAL, GAL1, GALN, GLNN
Sequence domains: Galanin
Guanine nucleotide-binding protein G(q) Chain: B
Molecule details ›
Chain: B
Length: 246 amino acids
Theoretical weight: 28.03 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: C
Molecule details ›
Chain: C
Length: 348 amino acids
Theoretical weight: 38.39 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: D
Molecule details ›
Chain: D
Length: 71 amino acids
Theoretical weight: 7.85 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
single Fab chain (svFv16) Chain: E
Molecule details ›
Chain: E
Length: 300 amino acids
Theoretical weight: 32.07 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Galanin receptor type 2 Chain: F
Molecule details ›
Chain: F
Length: 332 amino acids
Theoretical weight: 36.4 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O43603 (Residues: 1-314; Coverage: 81%)
Gene names: GALNR2, GALR2
Sequence domains: 7 transmembrane receptor (rhodopsin family)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-33230
Expression systems:
  • synthetic construct
  • Homo sapiens
  • Spodoptera frugiperda

Quick links

Citations

3 citation in other articles

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Chen et al. (2023)
2 more

1 mention without citation

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