› 7xke

Electron Microscopy
2.9Å resolution

Cryo-EM structure of DHEA-ADGRG2-FL-Gs complex

Released:
DOI: 10.2210/pdb7xke/pdb
PDB entry 7xke coloured by chain, front view.
PDB entry 7xke coloured by chain, side view.
PDB entry 7xke coloured by chain, top view.
Source organisms:
Primary publication:
Structures of the ADGRG2-Gs complex in apo and ligand-bound forms.
Lin H, Xiao P, Bu RQ, Guo S, Yang Z, Yuan D, Zhu ZL, Zhang CX, He QT, Zhang C, Ping YQ, Zhao RJ, Ma CS, Liu CH, Zhang XN, Jiang D, Huang S, Xi YT, Zhang DL, Xue CY, Yang BS, Li JY, Lin HC, Zeng XH, Zhao H, Xu WM, Yi F, Liu Z, Sun JP, Yu X
Nat Chem Biol 18 1196-1203 (2022)
PMID: 35982227
Related structures: EMD-33249

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-225736 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
mini-Gs Chain: A
Molecule details ›
Chain: A
Length: 361 amino acids
Theoretical weight: 41.88 KDa
Source organism: Homo sapiens
Expression system: Baculovirus expression vector pFastBac1-HM
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 358 amino acids
Theoretical weight: 39.49 KDa
Source organism: Homo sapiens
Expression system: Baculovirus expression vector pFastBac1-HM
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
NB35 Chain: N
Adhesion G-protein coupled receptor G2 Chain: R

Ligands and Environments

1 bound ligand:
Ligand AND 1 x AND
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-33249
Expression systems:
  • Baculovirus expression vector pFastBac1-HM
  • Escherichia coli

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Citations

1 review citation

Structure, function and drug discovery of GPCR signaling.
Cheng et al. (2023)
10 other articles