› 7xkr

Electron Microscopy
2.6Å resolution

F1 domain of FoF1-ATPase with the up form of epsilon subunit from Bacillus PS3

Released:
DOI: 10.2210/pdb7xkr/pdb
PDB entry 7xkr coloured by chain, front view.
PDB entry 7xkr coloured by chain, side view.
PDB entry 7xkr coloured by chain, top view.
Source organism: Bacillus sp. PS3
Primary publication:
Structural basis of unisite catalysis of bacterial F0F1-ATPase.
Nakano A, Kishikawa JI, Nakanishi A, Mitsuoka K, Yokoyama K
OpenAccess logo PNAS Nexus 1 pgac116 (2022)
PMID: 36741449
Related structures: EMD-33265

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102507 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
ATP synthase subunit alpha Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 502 amino acids
Theoretical weight: 54.85 KDa
Source organism: Bacillus sp. PS3
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: A0A0M3VGF9 (Residues: 1-502; Coverage: 100%)
Gene names: atpA, uncA
Sequence domains:
ATP synthase subunit beta Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 484 amino acids
Theoretical weight: 53.42 KDa
Source organism: Bacillus sp. PS3
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P07677 (Residues: 1-473; Coverage: 100%)
Gene name: atpD
Sequence domains:
ATP synthase gamma chain Chain: G
Molecule details ›
Chain: G
Length: 285 amino acids
Theoretical weight: 31.86 KDa
Source organism: Bacillus sp. PS3
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: A0A0M4TPJ7 (Residues: 1-285; Coverage: 100%)
Gene names: atpG, uncG
Sequence domains: ATP synthase
ATP synthase epsilon chain Chain: H
Molecule details ›
Chain: H
Length: 133 amino acids
Theoretical weight: 14.59 KDa
Source organism: Bacillus sp. PS3
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: A0A0M5MQR7 (Residues: 1-133; Coverage: 100%)
Gene names: atpC, uncC
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand ATP 4 x ATP
Ligand MG 5 x MG
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.6Å
Relevant EMDB volumes: EMD-33265
Expression system: Escherichia coli K-12

Quick links

Citations

4 citation in other articles

Beyond binding change: the molecular mechanism of ATP hydrolysis by F1-ATPase and its biochemical consequences.
Nath S. (2023)
3 more

1 mention without citation

Elucidating Events within the Black Box of Enzyme Catalysis in Energy Metabolism: Insights into the Molecular Mechanism of ATP Hydrolysis by F1-ATPase.
Nath S. (2023)