› 7xml

Electron Microscopy
3.2Å resolution

Cryo-EM structure of PEIP-Bs_enolase complex

Released:
DOI: 10.2210/pdb7xml/pdb
PDB entry 7xml coloured by chain, front view.
PDB entry 7xml coloured by chain, side view.
PDB entry 7xml coloured by chain, top view.
Source organisms:
Primary publication:
Bacteriophage protein PEIP is a potent Bacillus subtilis enolase inhibitor.
Zhang K, Li S, Wang Y, Wang Z, Mulvenna N, Yang H, Zhang P, Chen H, Li Y, Wang H, Gao Y, Wigneshweraraj S, Matthews S, Zhang K, Liu B
Cell Rep 40 111026 (2022)
PMID: 35793626
Related structures: EMD-33300

Function and Biology Details

Reaction catalysed: 
2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129232 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Enolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 430 amino acids
Theoretical weight: 46.63 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: P37869 (Residues: 1-430; Coverage: 100%)
Gene names: BSU33900, eno
Sequence domains:
Putative gene 60 protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 73 amino acids
Theoretical weight: 8.56 KDa
Source organism: Okubovirus SPO1
Expression system: Escherichia coli
UniProt:
  • Canonical: O48414 (Residues: 1-73; Coverage: 100%)
Gene name: 60

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-33300
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Beyond ENO1, emerging roles and targeting strategies of other enolases in cancers.
Ni et al. (2023)
2 other articles