› 7xmt

Electron Microscopy
2.8Å resolution

CryoEM structure of somatostatin receptor 4 (SSTR4) with Gi1 and J-2156

Released:
DOI: 10.2210/pdb7xmt/pdb
PDB entry 7xmt coloured by chain, front view.
PDB entry 7xmt coloured by chain, side view.
PDB entry 7xmt coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into ligand recognition and selectivity of somatostatin receptors.
Zhao W, Han S, Qiu N, Feng W, Lu M, Zhang W, Wang M, Zhou Q, Chen S, Xu W, Du J, Chu X, Yi C, Dai A, Hu L, Shen MY, Sun Y, Zhang Q, Ma Y, Zhong W, Yang D, Wang MW, Wu B, Zhao Q
OpenAccess logo Cell Res (2022)
PMID: 35739238
Related structures: EMD-33304

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-225590 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Somatostatin receptor type 4 Chain: R
Molecule details ›
Chain: R
Length: 375 amino acids
Theoretical weight: 40.67 KDa
Source organism: Homo sapiens
Expression system: Insecta environmental sample
UniProt:
  • Canonical: P31391 (Residues: 2-328; Coverage: 84%)
Gene name: SSTR4
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.45 KDa
Source organism: Homo sapiens
Expression system: Insecta environmental sample
UniProt:
  • Canonical: P63096 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
single chain variable fragment of antibody Chain: S
Molecule details ›
Chain: S
Length: 292 amino acids
Theoretical weight: 31.55 KDa
Source organism: Mus musculus
Expression system: Insecta environmental sample
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Insecta environmental sample
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 351 amino acids
Theoretical weight: 38.74 KDa
Source organism: Homo sapiens
Expression system: Insecta environmental sample
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat

Ligands and Environments

1 bound ligand:
Ligand I8B 1 x I8B
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-33304
Expression system: Insecta environmental sample

Quick links

Citations

2 review citations

CHARMM-GUI Membrane Builder: Past, Current, and Future Developments and Applications.
Feng et al. (2023)
1 more

1 mention without citation

Identification of a Novel SSTR3 Full Agonist for the Treatment of Nonfunctioning Pituitary Adenomas.
Modena et al. (2023)