› 7xow

Electron Microscopy
3.1Å resolution

Structural insights into human brain gut peptide cholecystokinin receptors

Released:
DOI: 10.2210/pdb7xow/pdb
PDB entry 7xow coloured by chain, front view.
PDB entry 7xow coloured by chain, side view.
PDB entry 7xow coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into human brain-gut peptide cholecystokinin receptors.
Ding Y, Zhang H, Liao YY, Chen LN, Ji SY, Qin J, Mao C, Shen DD, Lin L, Wang H, Zhang Y, Li XM
OpenAccess logo Cell Discov 8 55 (2022)
PMID: 35672283
Related structures: EMD-33361

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-246313 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Gastrin/cholecystokinin type B receptor Chain: R
Molecule details ›
Chain: R
Length: 447 amino acids
Theoretical weight: 48.47 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P32239 (Residues: 1-447; Coverage: 100%)
Gene names: CCKBR, CCKRB
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 345 amino acids
Theoretical weight: 37.92 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 70 amino acids
Theoretical weight: 7.73 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 2-71; Coverage: 99%)
Gene name: GNG2
Sequence domains: GGL domain
scfv16 Chain: E
Molecule details ›
Chain: E
Length: 269 amino acids
Theoretical weight: 28.81 KDa
Source organism: Mus musculus
Guanine nucleotide-binding protein G(i) subunit alpha-1; Guanine nucleotide-binding protein G(q) subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 353 amino acids
Theoretical weight: 41.24 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 30-30; Coverage: 0%)
  • Canonical: P50148 (Residues: 37-359; Coverage: 90%)
Gene names: GAQ, GNAI1, GNAQ
Sequence domains: G-protein alpha subunit
Gastrin Chain: L
Molecule details ›
Chain: L
Length: 17 amino acids
Theoretical weight: 2.2 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01350 (Residues: 76-92; Coverage: 21%)
Gene names: GAS, GAST
Sequence domains: Gastrin/cholecystokinin family

Ligands and Environments

No bound ligands
1 modified residue:
Ligand TYS 1 x TYS

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-33361
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

2 citation in other articles

A Novel CCK Receptor GPR173 Mediates Potentiation of GABAergic Inhibition.
He et al. (2023)
1 more