› 7xp4

Electron Microscopy
3.01Å resolution

Cryo-EM structure of a class T GPCR in apo state

Released:
DOI: 10.2210/pdb7xp4/pdb
PDB entry 7xp4 coloured by chain, front view.
PDB entry 7xp4 coloured by chain, side view.
PDB entry 7xp4 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for strychnine activation of human bitter taste receptor TAS2R46.
Xu W, Wu L, Liu S, Liu X, Cao X, Zhou C, Zhang J, Fu Y, Guo Y, Wu Y, Tan Q, Wang L, Liu J, Jiang L, Fan Z, Pei Y, Yu J, Cheng J, Zhao S, Hao X, Liu ZJ, Hua T
Science 377 1298-1304 (2022)
PMID: 36108005
Related structures: EMD-33364

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-225498 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 264 amino acids
Theoretical weight: 30.58 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63092 (Residues: 5-389; Coverage: 62%)
  • Best match: P63092-2 (Residues: 5-375)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 366 amino acids
Theoretical weight: 39.73 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Nanobody 35 Chain: N
Endoglucanase H; Taste receptor type 2 member 46 Chain: R

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.01Å
Relevant EMDB volumes: EMD-33364
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

5 review citations

G protein-coupled receptors (GPCRs): advances in structures, mechanisms, and drug discovery.
Zhang et al. (2024)
4 more

3 mentions without citation

Structure, function and drug discovery of GPCR signaling.
Cheng et al. (2023)
2 more