› 7xpq

X-ray diffraction
2.15Å resolution

Crystal Structure of UDP-Glc/GlcNAc 4-Epimerase with NAD/UDP-GlcNAc

Released:
DOI: 10.2210/pdb7xpq/pdb
PDB entry 7xpq coloured by chain, front view.
PDB entry 7xpq coloured by chain, side view.
PDB entry 7xpq coloured by chain, top view.
Source organism: Zea mays
Primary publication:
A maize epimerase modulates cell wall synthesis and glycosylation during stomatal morphogenesis.
Zhou Y, Zhang T, Wang X, Wu W, Xing J, Li Z, Qiao X, Zhang C, Wang X, Wang G, Li W, Bai S, Li Z, Suo Y, Wang J, Niu Y, Zhang J, Lan C, Hu Z, Li B, Zhang X, Wang W, Galbraith DW, Chen Y, Guo S, Song CP
OpenAccess logo Nat Commun 14 4384 (2023)
PMID: 37474494

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucose = UDP-alpha-D-galactose
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-215640 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-glucose 4-epimerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 355 amino acids
Theoretical weight: 38.97 KDa
Source organism: Zea mays
Expression system: Escherichia coli
UniProt:
  • Canonical: C0HI30 (Residues: 1-355; Coverage: 100%)
Gene names: LOC542127, ZEAMMB73_Zm00001d029151
Sequence domains: GDP-mannose 4,6 dehydratase

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
1 bound ligand:
Ligand UD1 2 x UD1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 65.873Å b: 84.945Å c: 73.436Å
α: 90° β: 110.69° γ: 90°
R-values:
R R work R free
0.2 0.197 0.25
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Cell wall anisotropy plays a key role in Zea mays stomatal complex movement: the possible role of the cell wall matrix.
Gkolemis et al. (2023)