› 7xpy

X-ray diffraction
2.35Å resolution

Crystal structure of USP7 in complex with its inhibitor

Released:
DOI: 10.2210/pdb7xpy/pdb
PDB entry 7xpy coloured by chain, front view.
PDB entry 7xpy coloured by chain, side view.
PDB entry 7xpy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Neuroinflammation inhibition by small-molecule targeting USP7 noncatalytic domain for neurodegenerative disease therapy.
Zhang XW, Feng N, Liu YC, Guo Q, Wang JK, Bai YZ, Ye XM, Yang Z, Yang H, Liu Y, Yang MM, Wang YH, Shi XM, Liu D, Tu PF, Zeng KW
OpenAccess logo Sci Adv 8 eabo0789 (2022)
PMID: 35947662

Function and Biology Details

Reaction catalysed: 
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187973 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin carboxyl-terminal hydrolase 7 Chain: A
Molecule details ›
Chain: A
Length: 548 amino acids
Theoretical weight: 64.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q93009 (Residues: 560-1102; Coverage: 49%)
Gene names: HAUSP, USP7
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand EIB 1 x EIB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 78.005Å b: 80.17Å c: 150.857Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.266
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Chronic Neuroinflammation and Cognitive Decline in Patients with Cardiac Disease: Evidence, Relevance, and Therapeutic Implications.
Traub et al. (2023)
4 more