› 7xra

X-ray diffraction
1.95Å resolution

Drimenyl diphosphate synthase D303A from Streptomyces showdoensis in complex with farnesyl diphosphate (FPP) and Mg2+

Released:
DOI: 10.2210/pdb7xra/pdb
PDB entry 7xra coloured by chain, front view.
PDB entry 7xra coloured by chain, side view.
PDB entry 7xra coloured by chain, top view.
Source organism: Streptomyces showdoensis
Primary publication:
Discovery, Structure, and Mechanism of a Class II Sesquiterpene Cyclase.
Pan X, Du W, Zhang X, Lin X, Li FR, Yang Q, Wang H, Rudolf JD, Zhang B, Dong LB
J Am Chem Soc 144 22067-22074 (2022)
PMID: 36416740

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104391 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Drimenyl diphosphate synthase Chain: A
Molecule details ›
Chain: A
Length: 514 amino acids
Theoretical weight: 55.04 KDa
Source organism: Streptomyces showdoensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A2P2GK84 (Residues: 16-523; Coverage: 95%)
Gene name: VO63_21045
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand FPP 1 x FPP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL02U1
Spacegroup: C2221
Unit cell:
a: 51.24Å b: 113.62Å c: 180.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.222
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Fungal BGCs for Production of Secondary Metabolites: Main Types, Central Roles in Strain Improvement, and Regulation According to the Piano Principle.
Zhgun AA. (2023)
2 other articles

1 mention without citation

Discovery, Structure, and Mechanism of a Class II Sesquiterpene Cyclase.
Pan et al. (2022)