› 7xs4

X-ray diffraction
1.85Å resolution

Crystal structure of URT1 in complex with AAAU RNA

Released:
DOI: 10.2210/pdb7xs4/pdb
PDB entry 7xs4 coloured by chain, front view.
PDB entry 7xs4 coloured by chain, side view.
PDB entry 7xs4 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Molecular mechanism underlying the di-uridylation activity of Arabidopsis TUTase URT1.
Hu Q, Yang H, Li M, Zhu L, Lv M, Li F, Zhang Z, Ren G, Gong Q
OpenAccess logo Nucleic Acids Res (2022)
PMID: 36177876

Function and Biology Details

Reaction catalysed: 
UTP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130278 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
UTP:RNA uridylyltransferase 1 Chain: A
Molecule details ›
Chain: A
Length: 364 amino acids
Theoretical weight: 42.34 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: O64642 (Residues: 410-764; Coverage: 47%)
Gene names: At2g45620, F17K2.15, URT1
Sequence domains:
RNA (5'-R(*AP*AP*AP*U)-3') Chain: B
Molecule details ›
Chain: B
Length: 4 nucleotides
Theoretical weight: 1.25 KDa
Source organism: Arabidopsis thaliana
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 63.164Å b: 63.264Å c: 81.387Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.173 0.202
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 citations in other articles

Mechanism of U6 snRNA oligouridylation by human TUT1.
Yamashita et al. (2023)

1 mention without citation

Molecular mechanism underlying the di-uridylation activity of Arabidopsis TUTase URT1.
Hu et al. (2022)