› 7xso

Electron Microscopy
3.01Å resolution

Structure of the type III-E CRISPR-Cas effector gRAMP

Released:
DOI: 10.2210/pdb7xso/pdb
PDB entry 7xso coloured by chain, front view.
PDB entry 7xso coloured by chain, side view.
PDB entry 7xso coloured by chain, top view.
Source organism: Candidatus Scalindua brodae
Primary publication:
Target RNA activates the protease activity of Craspase to confer antiviral defense.
Liu X, Zhang L, Wang H, Xiu Y, Huang L, Gao Z, Li N, Li F, Xiong W, Gao T, Zhang Y, Yang M, Feng Y
Mol Cell (2022)
PMID: 36306795
Related structures: EMD-33429

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100875 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RAMP superfamily protein Chain: A
Molecule details ›
Chain: A
Length: 1722 amino acids
Theoretical weight: 197.82 KDa
Source organism: Candidatus Scalindua brodae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0B0EGF3 (Residues: 1-1717; Coverage: 100%)
Gene name: SCABRO_02597
Sequence domains: RAMP superfamily
RNA (35-MER) Chain: D
Molecule details ›
Chain: D
Length: 74 nucleotides
Theoretical weight: 23.74 KDa
Source organism: Candidatus Scalindua brodae
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.01Å
Relevant EMDB volumes: EMD-33429
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Unity among the diverse RNA-guided CRISPR-Cas interference mechanisms.
Ganguly et al. (2024)
8 other articles

2 mentions without citation

Structure and the Mode of Activity of Lon Proteases from Diverse Organisms.
Wlodawer et al. (2022)
1 more