› 7xsr

Electron Microscopy
2.97Å resolution

Structure of Craspase-target RNA

Released:
DOI: 10.2210/pdb7xsr/pdb
PDB entry 7xsr coloured by chain, front view.
PDB entry 7xsr coloured by chain, side view.
PDB entry 7xsr coloured by chain, top view.
Source organism: Candidatus Scalindua brodae
Primary publication:
Target RNA activates the protease activity of Craspase to confer antiviral defense.
Liu X, Zhang L, Wang H, Xiu Y, Huang L, Gao Z, Li N, Li F, Xiong W, Gao T, Zhang Y, Yang M, Feng Y
Mol Cell (2022)
PMID: 36306795
Related structures: EMD-33432

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100868 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (4 distinct):
RAMP superfamily protein Chain: B
Molecule details ›
Chain: B
Length: 1722 amino acids
Theoretical weight: 197.82 KDa
Source organism: Candidatus Scalindua brodae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0B0EGF3 (Residues: 1-1717; Coverage: 100%)
Gene name: SCABRO_02597
Sequence domains: RAMP superfamily
CHAT domain-containing protein Chain: C
Molecule details ›
Chain: C
Length: 716 amino acids
Theoretical weight: 82.55 KDa
Source organism: Candidatus Scalindua brodae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0B0EKL4 (Residues: 1-716; Coverage: 100%)
Gene name: SCABRO_02601
Sequence domains: CHAT domain
RNA (5'-R(P*GP*GP*GP*GP*CP*AP*GP*AP*AP*AP*AP*UP*UP*GP*G)-3') Chain: A
Molecule details ›
Chain: A
Length: 38 nucleotides
Theoretical weight: 12.32 KDa
RNA (34-MER) Chain: D
Molecule details ›
Chain: D
Length: 74 nucleotides
Theoretical weight: 23.74 KDa

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.97Å
Relevant EMDB volumes: EMD-33432
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Unity among the diverse RNA-guided CRISPR-Cas interference mechanisms.
Ganguly et al. (2024)
8 other articles