› 7xt8

Electron Microscopy
3.1Å resolution

Serotonin 4 (5-HT4) receptor-Gs-Nb35 complex

Released:
DOI: 10.2210/pdb7xt8/pdb
PDB entry 7xt8 coloured by chain, front view.
PDB entry 7xt8 coloured by chain, side view.
PDB entry 7xt8 coloured by chain, top view.
Source organisms:
Primary publication:
GPCRs steer Gi and Gs selectivity via TM5-TM6 switches as revealed by structures of serotonin receptors.
Huang S, Xu P, Shen DD, Simon IA, Mao C, Tan Y, Zhang H, Harpsøe K, Li H, Zhang Y, You C, Yu X, Jiang Y, Zhang Y, Gloriam DE, Xu HE
Mol Cell (2022)
PMID: 35714614
Related structures: EMD-33442

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-532606 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 45.68 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 100%)
  • Best match: P63092-4 (Residues: 1-395)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 345 amino acids
Theoretical weight: 37.92 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody35 Chain: N
Molecule details ›
Chain: N
Length: 139 amino acids
Theoretical weight: 15.27 KDa
Source organism: Lama glama
Expression system: Escherichia coli
5-hydroxytryptamine receptor 4; Soluble cytochrome b562 Chain: R
Molecule details ›
Chain: R
Length: 527 amino acids
Theoretical weight: 59.53 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P0ABE7 (Residues: 23-127; Coverage: 99%)
  • Canonical: Q13639 (Residues: 2-388; Coverage: 100%)
Gene names: HTR4, cybC
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand CLR 5 x CLR
Ligand PLM 2 x PLM
Ligand SRO 1 x SRO
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-33442
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

8 review citations

New insights into GPCR coupling and dimerisation from cryo-EM structures.
Gusach et al. (2023)
7 more

9 mentions without citation

Structural studies of serotonin receptor family.
Parajulee et al. (2023)
8 more