› 7xtg

Electron Microscopy
2.2Å resolution

Cryo-EM structure of Listeria monocytogenes man-PTS complexed with pediocin PA-1

Released:
DOI: 10.2210/pdb7xtg/pdb
PDB entry 7xtg coloured by chain, front view.
PDB entry 7xtg coloured by chain, side view.
PDB entry 7xtg coloured by chain, top view.
Source organisms:
Primary publication:
Structural Basis of the Immunity Mechanisms of Pediocin-like Bacteriocins.
Zhu L, Zeng J, Wang J
Appl Environ Microbiol e0048122 (2022)
PMID: 35703550
Related structures: EMD-33448

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-235046 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Bacteriocin curvacin-A Chains: A, C, G
Molecule details ›
Chains: A, C, G
Length: 42 amino acids
Theoretical weight: 4.44 KDa
Source organism: Pediococcus acidilactici
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A311 (Residues: 19-59; Coverage: 70%)
Gene name: curA
Sequence domains: Class II bacteriocin
Sakacin-A immunity factor Chains: B, F, J
Molecule details ›
Chains: B, F, J
Length: 88 amino acids
Theoretical weight: 10.21 KDa
Source organism: Latilactobacillus sakei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q48864 (Residues: 3-90; Coverage: 98%)
Gene name: saiA
Mannose-specific phosphotransferase system, enzyme IIC Chains: D, H, Y
Molecule details ›
Chains: D, H, Y
Length: 248 amino acids
Theoretical weight: 25.46 KDa
Source organism: Listeria monocytogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: Q38YH6 (Residues: 2-249; Coverage: 93%)
Gene names: LCA_0450, manM
Sequence domains: PTS system sorbose-specific iic component
Mannose-specific phosphotransferase system, enzyme IID Chains: E, I, Z
Molecule details ›
Chains: E, I, Z
Length: 300 amino acids
Theoretical weight: 33 KDa
Source organism: Listeria monocytogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: Q38YH5 (Residues: 4-303; Coverage: 99%)
Gene names: LCA_0451, manN
Sequence domains: PTS system mannose/fructose/sorbose family IID component

Ligands and Environments

1 bound ligand:
Ligand MAN 3 x MAN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.2Å
Relevant EMDB volumes: EMD-33448
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Antimicrobial Activity and Immunomodulatory Properties of Acidocin A, the Pediocin-like Bacteriocin with the Non-Canonical Structure.
Antoshina et al. (2022)
2 more