› 7xur

Electron Microscopy
3.49Å resolution

The cryo-EM structure of human mini-SNAPc in complex with hU6-1 PSE

Released:
DOI: 10.2210/pdb7xur/pdb
PDB entry 7xur coloured by chain, front view.
PDB entry 7xur coloured by chain, side view.
PDB entry 7xur coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of human SNAPc recognizing proximal sequence element of snRNA promoter.
Sun J, Li X, Hou X, Cao S, Cao W, Zhang Y, Song J, Wang M, Wang H, Yan X, Li Z, Roeder RG, Wang W
OpenAccess logo Nat Commun 13 6871 (2022)
PMID: 36369505
Related structures: EMD-33477

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122315 (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
snRNA-activating protein complex subunit 4 Chain: A
Molecule details ›
Chain: A
Length: 522 amino acids
Theoretical weight: 60.78 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q5SXM2 (Residues: 1-505; Coverage: 34%)
Gene names: SNAP190, SNAPC4
Sequence domains: Myb-like DNA-binding domain
snRNA-activating protein complex subunit 3 Chain: B
Molecule details ›
Chain: B
Length: 411 amino acids
Theoretical weight: 46.81 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q92966 (Residues: 1-411; Coverage: 100%)
Gene names: SNAP50, SNAPC3
Sequence domains: snRNA-activating protein complex (SNAPc), subunit 3
snRNA-activating protein complex subunit 1 Chain: C
Molecule details ›
Chain: C
Length: 282 amino acids
Theoretical weight: 33.56 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q16533 (Residues: 1-268; Coverage: 73%)
Gene names: SNAP43, SNAPC1
Sequence domains: Small nuclear RNA activating complex (SNAPc), subunit 1
DNA (35-MER) Chain: X
Molecule details ›
Chain: X
Length: 35 nucleotides
Theoretical weight: 10.73 KDa
DNA (35-MER) Chain: Y
Molecule details ›
Chain: Y
Length: 35 nucleotides
Theoretical weight: 10.8 KDa

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.49Å
Relevant EMDB volumes: EMD-33477
Expression system: Spodoptera frugiperda

Quick links

Citations

1 review citation

Introns: the "dark matter" of the eukaryotic genome.
Girardini et al. (2023)
3 other articles