7xut

X-ray diffraction
1.6Å resolution

Crystal structure of RPA70N-WRN fusion

Released:
Model geometry
Fit model/data

Function and Biology Details

Reaction catalysed:
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-218080 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN; Replication protein A 70 kDa DNA-binding subunit, N-terminally processed Chains: A, B
Molecule details ›
Chains: A, B
Length: 140 amino acids
Theoretical weight: 15.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P27694 (Residues: 1-121; Coverage: 20%)
  • Canonical: Q14191 (Residues: 435-451; Coverage: 1%)
Gene names: RECQ3, RECQL2, REPA1, RPA1, RPA70, WRN
Sequence domains: Replication factor-A protein 1, N-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 32.936Å b: 58.609Å c: 111.786Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.179 0.214
Expression system: Escherichia coli