› 7xvg

Electron Microscopy
3.6Å resolution

Cryo-EM structure of binary complex of plant NLR Sr35 and effector AvrSr35

Released:
DOI: 10.2210/pdb7xvg/pdb
PDB entry 7xvg coloured by chain, front view.
PDB entry 7xvg coloured by chain, side view.
PDB entry 7xvg coloured by chain, top view.
Source organisms:
Primary publication:
Pathogen effector AvrSr35 triggers Sr35 resistosome assembly via a direct recognition mechanism.
Zhao YB, Liu MX, Chen TT, Ma X, Li ZK, Zheng Z, Zheng SR, Chen L, Li YZ, Tang LR, Chen Q, Wang P, Ouyang S
OpenAccess logo Sci Adv 8 eabq5108 (2022)
PMID: 36083908
Related structures: EMD-33486

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105315 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
CNL9 Chain: A
Molecule details ›
Chain: A
Length: 929 amino acids
Theoretical weight: 105.39 KDa
Source organism: Triticum monococcum
Expression system: Insect expression vector pBlueBacmsGCB1
UniProt:
  • Canonical: S5ABD6 (Residues: 1-919; Coverage: 100%)
Gene name: Sr35
Sequence domains:
Avirulence factor Chain: B
Molecule details ›
Chain: B
Length: 441 amino acids
Theoretical weight: 51.23 KDa
Source organism: Puccinia graminis f. sp. tritici
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A5B0N367 (Residues: 141-558; Coverage: 76%)
Gene names: PGT21_019944, PGTUg99_030428

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-33486
Expression systems:
  • Insect expression vector pBlueBacmsGCB1
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

5 review citations

NLR receptors in plant immunity: making sense of the alphabet soup.
Contreras et al. (2023)
4 more