› 7xw5

Electron Microscopy
2.96Å resolution

TSHR-thyroid stimulating hormone-Gs-ML109 complex

Released:
DOI: 10.2210/pdb7xw5/pdb
PDB entry 7xw5 coloured by chain, front view.
PDB entry 7xw5 coloured by chain, side view.
PDB entry 7xw5 coloured by chain, top view.
Source organisms:
Primary publication:
Hormone- and antibody-mediated activation of the thyrotropin receptor.
Duan J, Xu P, Luan X, Ji Y, He X, Song N, Yuan Q, Jin Y, Cheng X, Jiang H, Zheng J, Zhang S, Jiang Y, Xu HE
Nature (2022)
PMID: 35940204
Related structures: EMD-33491

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-224940 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 29.07 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63092 (Residues: 6-394; Coverage: 63%)
  • Best match: P63092-2 (Residues: 6-380)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 350 amino acids
Theoretical weight: 38.61 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody35 Chain: N
Molecule details ›
Chain: N
Length: 134 amino acids
Theoretical weight: 14.71 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Thyrotropin receptor Chain: R
Molecule details ›
Chain: R
Length: 702 amino acids
Theoretical weight: 79.92 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P16473 (Residues: 21-764; Coverage: 93%)
  • Best match: P16473-2 (Residues: 21-231)
Gene names: LGR3, TSHR
Sequence domains:
Glycoprotein hormones alpha chain Chain: X
Molecule details ›
Chain: X
Length: 92 amino acids
Theoretical weight: 10.22 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01215 (Residues: 25-116; Coverage: 100%)
Gene name: CGA
Sequence domains: Glycoprotein hormone
Thyrotropin subunit beta Chain: Y
Molecule details ›
Chain: Y
Length: 118 amino acids
Theoretical weight: 13.52 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01222 (Residues: 21-138; Coverage: 100%)
Gene name: TSHB
Sequence domains: Cystine-knot domain

Ligands and Environments

4 bound ligands:
Ligand NAG 7 x NAG
Ligand HOI 1 x HOI
Ligand CLR 14 x CLR
Ligand PLM 5 x PLM
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.96Å
Relevant EMDB volumes: EMD-33491
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli
  • Homo sapiens

Quick links

Citations

6 review citations

LGR4: A New Receptor Member in Endocrine and Metabolic Diseases.
Zhang et al. (2023)
5 more

1 mention without citation

Mechanism of hormone and allosteric agonist mediated activation of follicle stimulating hormone receptor.
Duan et al. (2023)