7xw6

Electron Microscopy
2.78Å resolution

TSHR-Gs-M22 antibody-ML109 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-276157 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 29.07 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63092 (Residues: 6-394; Coverage: 63%)
  • Best match: P63092-2 (Residues: 6-380)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 350 amino acids
Theoretical weight: 38.61 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
M22 antibody light chain Chain: L
Molecule details ›
Chain: L
Length: 112 amino acids
Theoretical weight: 11.97 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
Nanobody35 Chain: N
Molecule details ›
Chain: N
Length: 134 amino acids
Theoretical weight: 14.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Thyrotropin receptor Chain: R
Molecule details ›
Chain: R
Length: 702 amino acids
Theoretical weight: 79.92 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P16473 (Residues: 21-764; Coverage: 93%)
  • Best match: P16473-2 (Residues: 21-231)
Gene names: LGR3, TSHR
Sequence domains:
M22 antibody heavy chain Chain: Y
Molecule details ›
Chain: Y
Length: 120 amino acids
Theoretical weight: 13.4 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.78Å
Relevant EMDB volumes: EMD-33492
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli