› 7xw9

Electron Microscopy
2.7Å resolution

Cryo-EM structure of the TRH-bound human TRHR-Gq complex

Released:
DOI: 10.2210/pdb7xw9/pdb
PDB entry 7xw9 coloured by chain, front view.
PDB entry 7xw9 coloured by chain, side view.
PDB entry 7xw9 coloured by chain, top view.
Source organisms:
Primary publication:
Molecular basis for the activation of thyrotropin-releasing hormone receptor.
Ji SY, Dong YJ, Chen LN, Zang SK, Wang J, Shen DD, Guo J, Qin J, Zhang H, Wang WW, Shen Q, Zhang Y, Song Z, Mao C
OpenAccess logo Cell Discov 8 116 (2022)
PMID: 36280665
Related structures: EMD-33494

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-276200 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Thyrotropin-releasing hormone receptor Chain: R
Molecule details ›
Chain: R
Length: 398 amino acids
Theoretical weight: 45.12 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(q) subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 409 amino acids
Theoretical weight: 46.96 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
single Fab chain (svFv16) Chain: S
TRH peptide Chain: L

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
Resolution: 2.7Å
Relevant EMDB volumes: EMD-33494
Expression systems:
  • Spodoptera frugiperda
  • Trichoplusia ni
  • Not provided

Quick links

Citations

1 review citation

Molecular regulation of calcium-sensing receptor (CaSR)-mediated signaling.
Tian et al. (2024)