7xwi

X-ray diffraction
2.22Å resolution

structure of patulin-detoxifying enzyme with NADPH

Released:

Function and Biology Details

Reaction catalysed:
A primary alcohol + NAD(+) = an aldehyde + NADH
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-105973 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Short-chain dehydrogenase/reductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 261 amino acids
Theoretical weight: 28.07 KDa
Source organism: Meyerozyma guilliermondii
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A888VSF1 (Residues: 1-250; Coverage: 100%)
Gene name: SDR
Sequence domains: Enoyl-(Acyl carrier protein) reductase

Ligands and Environments


Cofactor: Ligand NDP 4 x NDP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER METALJET
Spacegroup: P21
Unit cell:
a: 85.014Å b: 64.818Å c: 88.812Å
α: 90° β: 107.17° γ: 90°
R-values:
R R work R free
0.202 0.198 0.277
Expression system: Escherichia coli