› 7xy6

Electron Microscopy
2.99Å resolution

Adenosine receptor bound to an agonist in complex with G protein obtained by cryo-EM

Released:
DOI: 10.2210/pdb7xy6/pdb
PDB entry 7xy6 coloured by chain, front view.
PDB entry 7xy6 coloured by chain, side view.
PDB entry 7xy6 coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structure of the human adenosine A2B receptor-Gs signaling complex.
Chen Y, Zhang J, Weng Y, Xu Y, Lu W, Liu W, Liu M, Hua T, Song G
OpenAccess logo Sci Adv 8 eadd3709 (2022)
PMID: 36563137
Related structures: EMD-33512

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-232788 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 243 amino acids
Theoretical weight: 28.15 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63092 (Residues: 151-190, 204-394; Coverage: 55%)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 366 amino acids
Theoretical weight: 39.73 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 73 amino acids
Theoretical weight: 7.89 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-67; Coverage: 94%)
Gene name: GNG2
Sequence domains: GGL domain
Adenosine receptor A2b; Soluble cytochrome b562 Chain: R
Molecule details ›
Chain: R
Length: 628 amino acids
Theoretical weight: 69.59 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P0ABE7 (Residues: 23-127; Coverage: 99%)
  • Canonical: P29275 (Residues: 2-332; Coverage: 100%)
Gene names: ADORA2B, cybC
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand I5D 1 x I5D
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.99Å
Relevant EMDB volumes: EMD-33512
Expression system: Spodoptera frugiperda

Quick links

Citations

1 review citation

New paradigms in purinergic receptor ligand discovery.
Jacobson et al. (2023)
4 other articles

1 mention without citation

Cryo-EM structure of the human adenosine A2B receptor-Gs signaling complex.
Chen et al. (2022)