› 7xz6

Electron Microscopy
2.8Å resolution

GPR119-Gs-APD668 complex

Released:
DOI: 10.2210/pdb7xz6/pdb
PDB entry 7xz6 coloured by chain, front view.
PDB entry 7xz6 coloured by chain, side view.
PDB entry 7xz6 coloured by chain, top view.
Source organisms:
Primary publication:
Structural identification of lysophosphatidylcholines as activating ligands for orphan receptor GPR119.
Xu P, Huang S, Guo S, Yun Y, Cheng X, He X, Cai P, Lan Y, Zhou H, Jiang H, Jiang Y, Xie X, Xu HE
Nat Struct Mol Biol (2022)
PMID: 35970999
Related structures: EMD-33526

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-225136 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 45.7 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 100%)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 345 amino acids
Theoretical weight: 37.92 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody35 Chain: N
Molecule details ›
Chain: N
Length: 139 amino acids
Theoretical weight: 15.27 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Glucose-dependent insulinotropic receptor; Soluble cytochrome b562 Chain: R
Molecule details ›
Chain: R
Length: 485 amino acids
Theoretical weight: 53.79 KDa
Source organisms: Expression system: Trichoplusia ni
UniProt:
  • Canonical: P0ABE7 (Residues: 23-127; Coverage: 99%)
  • Canonical: Q8TDV5 (Residues: 1-335; Coverage: 100%)
Gene names: GPR119, cybC
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand I7J 1 x I7J
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-33526
Expression systems:
  • Trichoplusia ni
  • Escherichia coli

Quick links

Citations

3 review citations

Orphan G protein-coupled receptors: the ongoing search for a home.
Jobe et al. (2024)
2 more