› 7y27

Electron Microscopy
3.48Å resolution

Cryo-EM structure of the SST-14-bound SSTR2-miniGq-scFv16 complex

Released:
DOI: 10.2210/pdb7y27/pdb
PDB entry 7y27 coloured by chain, front view.
PDB entry 7y27 coloured by chain, side view.
PDB entry 7y27 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for the selective G protein signaling of somatostatin receptors.
Chen S, Teng X, Zheng S
Nat Chem Biol (2022)
PMID: 36138141
Related structures: EMD-33587

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-225582 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: A
Molecule details ›
Chain: A
Length: 338 amino acids
Theoretical weight: 37.2 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 3-340; Coverage: 99%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Engineered Guanine nucleotide-binding protein G(q) subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 243 amino acids
Theoretical weight: 27.81 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
single Fab chain (svFv16) Chain: D
Molecule details ›
Chain: D
Length: 258 amino acids
Theoretical weight: 27.71 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Somatostatin receptor type 2 Chain: E
Molecule details ›
Chain: E
Length: 337 amino acids
Theoretical weight: 37.76 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P30874 (Residues: 1-337; Coverage: 91%)
Gene name: SSTR2
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: F
Molecule details ›
Chain: F
Length: 71 amino acids
Theoretical weight: 7.85 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Somatostatin-14 Chain: C
Molecule details ›
Chain: C
Length: 12 amino acids
Theoretical weight: 1.51 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P61278 (Residues: 105-116; Coverage: 13%)
Gene name: SST
Sequence domains: Somatostatin/Cortistatin family

Ligands and Environments

1 bound ligand:
Ligand CLR 1 x CLR
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.48Å
Relevant EMDB volumes: EMD-33587
Expression systems:
  • Trichoplusia ni
  • Homo sapiens
  • Not provided

Quick links

Citations

1 review citation

Allosteric Regulation of G-Protein-Coupled Receptors: From Diversity of Molecular Mechanisms to Multiple Allosteric Sites and Their Ligands.
Shpakov AO. (2023)
3 other articles

1 mention without citation

Identification of a Novel SSTR3 Full Agonist for the Treatment of Nonfunctioning Pituitary Adenomas.
Modena et al. (2023)