7y38

Electron Microscopy
2.8Å resolution

Molecular architecture of the chikungunya virus replication complex

Released:

Function and Biology Details

Reactions catalysed:
ATP + RNA(n) = diphosphate + RNA(n+1)
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
ADP-D-ribose 1''-phosphate + H(2)O = ADP-D-ribose + phosphate
NTP + H(2)O = NDP + phosphate
A 5'-triphospho-[mRNA] + H(2)O = a 5'-diphospho-[mRNA] + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
PDBe Complex ID:
PDB-CPX-221653 (preferred)
Entry contents:
3 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
mRNA-capping enzyme nsP1 Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 573 amino acids
Theoretical weight: 64.33 KDa
Source organism: Chikungunya virus strain S27-African prototype
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8JUX6 (Residues: 1-516, 517-534; Coverage: 22%)
Sequence domains: Viral methyltransferase
RNA-directed RNA polymerase nsP4 Chain: X
Molecule details ›
Chain: X
Length: 611 amino acids
Theoretical weight: 68.37 KDa
Source organism: Onyong-nyong virus
Expression system: Escherichia coli
UniProt:
  • Canonical: O90368 (Residues: 1903-2513; Coverage: 24%)
Sequence domains: RNA dependent RNA polymerase
Protease nsP2 Chain: Y
RNA (5'-R(P*CP*CP*A)-3') Chain: Z

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-33591
Expression systems:
  • Homo sapiens
  • Escherichia coli