› 7y3f

Electron Microscopy
2.62Å resolution

Structure of the Anabaena PSI-monomer-IsiA supercomplex

Released:
DOI: 10.2210/pdb7y3f/pdb
PDB entry 7y3f coloured by chain, front view.
PDB entry 7y3f coloured by chain, side view.
PDB entry 7y3f coloured by chain, top view.
Source organism: Nostoc sp.
Primary publication:
Structure of a monomeric photosystem I core associated with iron-stress-induced-A proteins from Anabaena sp. PCC 7120.
Nagao R, Kato K, Hamaguchi T, Ueno Y, Tsuboshita N, Shimizu S, Furutani M, Ehira S, Nakajima Y, Kawakami K, Suzuki T, Dohmae N, Akimoto S, Yonekura K, Shen JR
Nat Commun 14 920 (2023)
PMID: 36805598
Related structures: EMD-33593

Function and Biology Details

Reaction catalysed: 
Reduced plastocyanin + oxidized ferredoxin + light = oxidized plastocyanin + reduced ferredoxin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptadecamer (preferred)
PDBe Complex ID:
PDB-CPX-227655 (preferred)
Entry contents:
14 distinct polypeptide molecules
Macromolecules (14 distinct):
Photosystem I P700 chlorophyll a apoprotein A1 Chain: A
Molecule details ›
Chain: A
Length: 752 amino acids
Theoretical weight: 83.29 KDa
Source organism: Nostoc sp.
UniProt:
  • Canonical: P58576 (Residues: 1-752; Coverage: 100%)
Gene names: alr5154, psaA
Sequence domains: Photosystem I psaA/psaB protein
Photosystem I P700 chlorophyll a apoprotein A2 1 Chain: B
Molecule details ›
Chain: B
Length: 741 amino acids
Theoretical weight: 83.46 KDa
Source organism: Nostoc sp.
UniProt:
  • Canonical: P58565 (Residues: 1-741; Coverage: 100%)
Gene names: alr5155, psaB1
Sequence domains: Photosystem I psaA/psaB protein
Photosystem I iron-sulfur center Chain: C
Molecule details ›
Chain: C
Length: 81 amino acids
Theoretical weight: 8.83 KDa
Source organism: Nostoc sp.
UniProt:
  • Canonical: P0A410 (Residues: 1-81; Coverage: 100%)
Gene names: asr3463, psaC
Sequence domains: 4Fe-4S dicluster domain
Photosystem I reaction center subunit II Chain: D
Molecule details ›
Chain: D
Length: 139 amino acids
Theoretical weight: 15.18 KDa
Source organism: Nostoc sp.
UniProt:
  • Canonical: P58573 (Residues: 1-139; Coverage: 100%)
Gene names: all0329, psaD
Sequence domains: PsaD
Photosystem I reaction center subunit IV Chain: E
Molecule details ›
Chain: E
Length: 70 amino acids
Theoretical weight: 7.9 KDa
Source organism: Nostoc sp.
UniProt:
  • Canonical: P58575 (Residues: 1-70; Coverage: 100%)
Gene names: asr4319, psaE
Sequence domains: Photosystem I reaction centre subunit IV / PsaE
Photosystem I reaction center subunit III Chain: F
Molecule details ›
Chain: F
Length: 164 amino acids
Theoretical weight: 17.85 KDa
Source organism: Nostoc sp.
UniProt:
  • Canonical: P58564 (Residues: 1-164; Coverage: 100%)
Gene names: all0109, psaF
Sequence domains: Photosystem I reaction centre subunit III
Photosystem I reaction center subunit VIII Chain: I
Molecule details ›
Chain: I
Length: 46 amino acids
Theoretical weight: 5.07 KDa
Source organism: Nostoc sp.
UniProt:
  • Canonical: P58560 (Residues: 1-46; Coverage: 100%)
Gene names: asl3849, psaI
Sequence domains: Photosystem I reaction centre subunit VIII
Photosystem I reaction center subunit IX Chain: J
Molecule details ›
Chain: J
Length: 49 amino acids
Theoretical weight: 5.5 KDa
Source organism: Nostoc sp.
UniProt:
  • Canonical: P58568 (Residues: 1-49; Coverage: 100%)
Gene names: asl0108, psaJ
Sequence domains: Photosystem I reaction centre subunit IX / PsaJ
Unknown Chain: K
Molecule details ›
Chain: K
Length: 82 amino acids
Theoretical weight: 7 KDa
Source organism: Nostoc sp.
Photosystem I reaction center subunit XII Chain: M
Molecule details ›
Chain: M
Length: 40 amino acids
Theoretical weight: 4.42 KDa
Source organism: Nostoc sp.
UniProt:
  • Canonical: Q8YNB0 (Residues: 1-32; Coverage: 100%)
Gene names: asr4657, psaM
Sequence domains: Photosystem I protein M (PsaM)
Photosystem I 4.8 kDa protein Chain: X
Molecule details ›
Chain: X
Length: 44 amino acids
Theoretical weight: 4.87 KDa
Source organism: Nostoc sp.
UniProt:
  • Canonical: P58566 (Residues: 1-44; Coverage: 100%)
Gene names: asr1283, psaX
Sequence domains: PsaX family
Photosystem I reaction center subunit XI Chain: 1
Molecule details ›
Chain: 1
Length: 476 amino acids
Theoretical weight: 51.72 KDa
Source organism: Nostoc sp.
UniProt:
  • Canonical: Q8YQ34 (Residues: 1-476; Coverage: 100%)
Gene names: all4002, psaL
Sequence domains:
IsiA Chains: 2, 3, 6
Molecule details ›
Chains: 2, 3, 6
Length: 325 amino acids
Theoretical weight: 27.68 KDa
Source organism: Nostoc sp.
Iron stress-induced chlorophyll-binding protein Chains: 4, 5
Molecule details ›
Chains: 4, 5
Length: 344 amino acids
Theoretical weight: 37.7 KDa
Source organism: Nostoc sp.
UniProt:
  • Canonical: Q8YQ35 (Residues: 1-344; Coverage: 100%)
Gene names: all4001, isiA
Sequence domains: Photosystem II protein

Ligands and Environments

8 bound ligands:
Ligand CL0 1 x CL0
Ligand CLA 155 x CLA
Ligand PQN 2 x PQN
Ligand SF4 3 x SF4
Ligand BCR 29 x BCR
Ligand LHG 4 x LHG
Ligand LMT 6 x LMT
Ligand LMG 1 x LMG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.62Å
Relevant EMDB volumes: EMD-33593

Quick links

Citations

1 review citation

High-Resolution Frequency-Domain Spectroscopic and Modeling Studies of Photosystem I (PSI), PSI Mutants and PSI Supercomplexes.
Zazubovich et al. (2024)
1 other article