7y3g

Electron Microscopy
2.77Å resolution

Cryo-EM structure of a class A orphan GPCR

Released:
Source organisms:
Primary publication:
Structural insight into the constitutive activity of human orphan receptor GPR12.
Sci Bull (Beijing) 68 95-104 (2023)
PMID: 36593162
Related structures: EMD-33594

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-225655 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 45.68 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 340 amino acids
Theoretical weight: 37.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Nanobody 35 Chain: N
G-protein coupled receptor 12 Chain: R

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.77Å
Relevant EMDB volumes: EMD-33594
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli