› 7y47

X-ray diffraction
2.5Å resolution

Crystal structure of bifunctional miltiradiene synthase from selaginella moellendorffii that complexed with GGPP

Released:
DOI: 10.2210/pdb7y47/pdb
PDB entry 7y47 coloured by chain, front view.
PDB entry 7y47 coloured by chain, side view.
PDB entry 7y47 coloured by chain, top view.
Source organism: Selaginella moellendorffii
Primary publication:
Structural and mechanistic insights into the precise product synthesis by a bifunctional miltiradiene synthase.
Tong Y, Ma X, Hu T, Chen K, Cui G, Su P, Xu H, Gao W, Jiang T, Huang L
OpenAccess logo Plant Biotechnol J (2022)
PMID: 36161753

Function and Biology Details

Reactions catalysed: 
(+)-copalyl diphosphate = miltiradiene + diphosphate
Geranylgeranyl diphosphate = (+)-copalyl diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124681 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional diterpene synthase, chloroplastic Chain: B
Molecule details ›
Chain: B
Length: 787 amino acids
Theoretical weight: 91.54 KDa
Source organism: Selaginella moellendorffii
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: G9MAN7 (Residues: 90-867; Coverage: 90%)
Gene names: MDS, SELMODRAFT_450918
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand IMD 1 x IMD
Ligand GRG 1 x GRG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 55.859Å b: 83.971Å c: 192.535Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.186 0.238
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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Citations

2 citation in other articles

Miltiradiene Production by Cytoplasmic Metabolic Engineering in Nicotiana benthamiana.
Ren et al. (2023)
1 more