› 7y5k

X-ray diffraction
3.48Å resolution

Crystal structure of human CAF-1 core complex in spacegroup C2221

Released:
DOI: 10.2210/pdb7y5k/pdb
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PDB entry 7y5k coloured by chain, front view.
PDB entry 7y5k coloured by chain, side view.
PDB entry 7y5k coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into histone binding and nucleosome assembly by chromatin assembly factor-1.
Liu CP, Yu Z, Xiong J, Hu J, Song A, Ding D, Yu C, Yang N, Wang M, Yu J, Hou P, Zeng K, Li Z, Zhang Z, Zhang X, Li W, Zhang Z, Zhu B, Li G, Xu RM
Science 381 eadd8673 (2023)
PMID: 37616371

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-226952 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Chromatin assembly factor 1 subunit A Chain: A
Molecule details ›
Chain: A
Length: 273 amino acids
Theoretical weight: 31.3 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q13111 (Residues: 442-714; Coverage: 29%)
Gene names: CAF, CAF1P150, CHAF1A
Sequence domains: Chromatin assembly factor 1 subunit A
Histone-binding protein RBBP4 Chain: C
Molecule details ›
Chain: C
Length: 425 amino acids
Theoretical weight: 47.71 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q09028 (Residues: 1-425; Coverage: 100%)
Gene names: RBAP48, RBBP4
Sequence domains:
Chromatin assembly factor 1 subunit B Chain: B

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: C2221
Unit cell:
a: 108.87Å b: 169.843Å c: 153.314Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.225 0.257
Expression system: Spodoptera frugiperda

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