› 7y6i

Electron Microscopy
2.85Å resolution

Cryo-EM structure of human sodium-chloride cotransporter

Released:
DOI: 10.2210/pdb7y6i/pdb
PDB entry 7y6i coloured by chain, front view.
PDB entry 7y6i coloured by chain, side view.
PDB entry 7y6i coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM structure of the human sodium-chloride cotransporter NCC.
Nan J, Yuan Y, Yang X, Shan Z, Liu H, Wei F, Zhang W, Zhang Y
OpenAccess logo Sci Adv 8 eadd7176 (2022)
PMID: 36351028
Related structures: EMD-33641

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157149 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Solute carrier family 12 member 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 1053 amino acids
Theoretical weight: 116.92 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P55017 (Residues: 1-1021; Coverage: 100%)
Gene names: NCC, SLC12A3, TSC
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
6 bound ligands:
Ligand CL 4 x CL
Ligand NA 4 x NA
Ligand Y01 6 x Y01
Ligand LPE 8 x LPE
Ligand POV 9 x POV
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.85Å
Relevant EMDB volumes: EMD-33641
Expression system: Homo sapiens

Quick links

Citations

5 review citations

Structural Pharmacology of Cation-Chloride Cotransporters.
Zhao et al. (2022)
4 more