› 7y9h

X-ray diffraction
2.03Å resolution

Crystal structure of diterpene synthase VenA from Streptomyces venezuelae ATCC 15439

Released:
DOI: 10.2210/pdb7y9h/pdb
PDB entry 7y9h coloured by chain, front view.
PDB entry 7y9h coloured by chain, side view.
PDB entry 7y9h coloured by chain, top view.
Source organism: Streptomyces venezuelae
Primary publication:
Molecular insights into the catalytic promiscuity of a bacterial diterpene synthase.
Li Z, Zhang L, Xu K, Jiang Y, Du J, Zhang X, Meng LH, Wu Q, Du L, Li X, Hu Y, Xie Z, Jiang X, Tang YJ, Wu R, Guo RT, Li S
OpenAccess logo Nat Commun 14 4001 (2023)
PMID: 37414771

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-217615 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Diterpene synthase VenA Chains: A, B
Molecule details ›
Chains: A, B
Length: 377 amino acids
Theoretical weight: 41.59 KDa
Source organism: Streptomyces venezuelae
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

3 bound ligands:
Ligand NA 3 x NA
Ligand EDO 7 x EDO
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER METALJET
Spacegroup: P32
Unit cell:
a: 93.999Å b: 93.999Å c: 80.747Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.162 0.16 0.206
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

Molecular insights into the catalytic promiscuity of a bacterial diterpene synthase.
Li et al. (2023)