› 7ydj

Electron Microscopy
3.03Å resolution

Cryo EM structure of CD97/miniG12 complex

Released:
DOI: 10.2210/pdb7ydj/pdb
PDB entry 7ydj coloured by chain, front view.
PDB entry 7ydj coloured by chain, side view.
PDB entry 7ydj coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: He Y, Wang N
Related structures: EMD-33749

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-235833 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1; Guanine nucleotide-binding protein subunit alpha-12 Chain: A
Molecule details ›
Chain: A
Length: 345 amino acids
Theoretical weight: 39.96 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 1-20, 60-181, 229-242; Coverage: 41%)
  • Canonical: Q03113 (Residues: 45-83, 209-255, 270-381; Coverage: 52%)
Gene names: GNA12, GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 345 amino acids
Theoretical weight: 37.92 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
scFv16 Chain: E
Molecule details ›
Chain: E
Length: 247 amino acids
Theoretical weight: 26.29 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Adhesion G protein-coupled receptor E5 subunit beta Chain: R
Molecule details ›
Chain: R
Length: 307 amino acids
Theoretical weight: 34.55 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P48960 (Residues: 530-835; Coverage: 38%)
Gene names: ADGRE5, CD97
Sequence domains: 7 transmembrane receptor (Secretin family)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.03Å
Relevant EMDB volumes: EMD-33749
Expression system: Spodoptera frugiperda

Quick links