› 7ydp

Electron Microscopy
3.1Å resolution

Cryo-EM structure of CD97/miniGs complex

Released:
DOI: 10.2210/pdb7ydp/pdb
PDB entry 7ydp coloured by chain, front view.
PDB entry 7ydp coloured by chain, side view.
PDB entry 7ydp coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of CD97 activation and G-protein coupling.
Wang N, Qian Y, Xia R, Zhu X, Xiong Y, Zhang A, Guo C, He Y
Cell Chem Biol (2023)
PMID: 37673067
Related structures: EMD-33755

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-235832 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Adhesion G protein-coupled receptor E5 subunit beta Chain: R
Molecule details ›
Chain: R
Length: 307 amino acids
Theoretical weight: 34.55 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P48960 (Residues: 530-835; Coverage: 38%)
Gene names: ADGRE5, CD97
Sequence domains: 7 transmembrane receptor (Secretin family)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Guanine nucleotide-binding protein G(i) subunit alpha-1; Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 361 amino acids
Theoretical weight: 41.88 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63092 (Residues: 1-3, 27-67, 205-251, 266-394; Coverage: 56%)
  • Canonical: P63096 (Residues: 4-19, 61-181, 229-242; Coverage: 40%)
Gene names: GNAI1, GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Nb35 Chain: D
Molecule details ›
Chain: D
Length: 161 amino acids
Theoretical weight: 17.38 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 345 amino acids
Theoretical weight: 37.92 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-33755
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

1 citations in other articles

Heterogeneity of tethered agonist signaling in adhesion G protein-coupled receptors.
Dates et al. (2024)