› 7ye4

Electron Microscopy
3.4Å resolution

BAM-EspP complex structure with BamA-G431C and G781C/EspP-N1293C and A1043C mutations in nanodisc

Released:
DOI: 10.2210/pdb7ye4/pdb
PDB entry 7ye4 coloured by chain, front view.
PDB entry 7ye4 coloured by chain, side view.
PDB entry 7ye4 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of BAM-mediated outer membrane β-barrel protein assembly.
Shen C, Chang S, Luo Q, Chan KC, Zhang Z, Luo B, Xie T, Lu G, Zhu X, Wei X, Dong C, Zhou R, Zhang X, Tang X, Dong H
Nature 617 185-193 (2023)
PMID: 37100902
Related structures: EMD-33763

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-216277 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Outer membrane protein assembly factor BamA Chain: A
Molecule details ›
Chain: A
Length: 810 amino acids
Theoretical weight: 90.74 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P0A940 (Residues: 1-810; Coverage: 100%)
Gene names: JW0172, b0177, bamA, yaeT, yzzN, yzzY
Sequence domains:
Outer membrane protein assembly factor BamB Chain: B
Molecule details ›
Chain: B
Length: 392 amino acids
Theoretical weight: 41.92 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P77774 (Residues: 1-392; Coverage: 100%)
Gene names: JW2496, b2512, bamB, yfgL
Sequence domains:
Outer membrane protein assembly factor BamC Chain: C
Molecule details ›
Chain: C
Length: 344 amino acids
Theoretical weight: 36.88 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P0A903 (Residues: 1-344; Coverage: 100%)
Gene names: JW2462, b2477, bamC, dapX, nlpB
Sequence domains: NlpB/DapX lipoprotein
Outer membrane protein assembly factor BamD Chain: D
Molecule details ›
Chain: D
Length: 245 amino acids
Theoretical weight: 27.86 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P0AC02 (Residues: 1-245; Coverage: 100%)
Gene names: JW2577, b2595, bamD, yfiO
Sequence domains: Outer membrane lipoprotein
Outer membrane protein assembly factor BamE Chain: E
Molecule details ›
Chain: E
Length: 123 amino acids
Theoretical weight: 13.53 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P0A937 (Residues: 1-113; Coverage: 100%)
Gene names: JW2598, b2617, bamE, smpA
Sequence domains: SmpA / OmlA family
Autotransporter protein EspP translocator Chain: P
Molecule details ›
Chain: P
Length: 353 amino acids
Theoretical weight: 38.76 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q7BSW5 (Residues: 948-1300; Coverage: 28%)
Gene names: ECO57PM78, L7020, espP
Sequence domains: Autotransporter beta-domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-33763
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

2 review citations

ATP-independent assembly machinery of bacterial outer membranes: BAM complex structure and function set the stage for next-generation therapeutics.
George et al. (2024)
1 more