› 7yfz

Electron Microscopy
3.19Å resolution

Cyanophage Pam3 baseplate proteins

Released:
DOI: 10.2210/pdb7yfz/pdb
PDB entry 7yfz coloured by chain, front view.
PDB entry 7yfz coloured by chain, side view.
PDB entry 7yfz coloured by chain, top view.
Source organism: uncultured cyanophage
Primary publication:
Fine structure and assembly pattern of a minimal myophage Pam3.
Yang F, Jiang YL, Zhang JT, Zhu J, Du K, Yu RC, Wei ZL, Kong WW, Cui N, Li WF, Chen Y, Li Q, Zhou CZ
OpenAccess logo Proc Natl Acad Sci U S A 120 e2213727120 (2023)
PMID: 36656854
Related structures: EMD-33802

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 42-mer (preferred)
PDBe Complex ID:
PDB-CPX-243039 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
Baseplate protein J-like domain-containing protein Chains: A, B, E, F, H, I, K, L, O, P, Q, R
Molecule details ›
Chains: A, B, E, F, H, I, K, L, O, P, Q, R
Length: 299 amino acids
Theoretical weight: 32.69 KDa
Source organism: uncultured cyanophage
UniProt:
  • Canonical: A0A9E7DT34 (Residues: 1-299; Coverage: 100%)
Gene name: Pam3_22
DUF2612 domain-containing protein Chains: C, D, G, J, M, S
Molecule details ›
Chains: C, D, G, J, M, S
Length: 238 amino acids
Theoretical weight: 26.19 KDa
Source organism: uncultured cyanophage
UniProt:
  • Canonical: A0A9E7DTG9 (Residues: 1-238; Coverage: 100%)
Gene name: Pam3_23
Sequence domains: Protein of unknown function (DUF2612)
DUF2634 domain-containing protein Chains: X, n, o, p, q, r
Molecule details ›
Chains: X, n, o, p, q, r
Length: 125 amino acids
Theoretical weight: 13.89 KDa
Source organism: uncultured cyanophage
UniProt:
  • Canonical: A0A9E7DT65 (Residues: 1-125; Coverage: 100%)
Gene name: Pam3_21
Dit-like phage tail protein N-terminal domain-containing protein Chains: a, b, c, d, e, f
Molecule details ›
Chains: a, b, c, d, e, f
Length: 191 amino acids
Theoretical weight: 19.85 KDa
Source organism: uncultured cyanophage
UniProt:
  • Canonical: A0A9E7DTH1 (Residues: 1-191; Coverage: 100%)
Gene name: Pam3_17
Sequence domains: Dit-like phage tail protein
Cyanophage baseplate Pam3 plug gp18 domain-containing protein Chains: h, i, j, k, l, m
Molecule details ›
Chains: h, i, j, k, l, m
Length: 106 amino acids
Theoretical weight: 11.72 KDa
Source organism: uncultured cyanophage
UniProt:
  • Canonical: A0A9E7DT67 (Residues: 1-106; Coverage: 100%)
Gene name: Pam3_18
Sequence domains: Domain of unknown function (DUF6983)
Hub protein Chains: t, u, v
Molecule details ›
Chains: t, u, v
Length: 229 amino acids
Theoretical weight: 25.2 KDa
Source organism: uncultured cyanophage
UniProt:
  • Canonical: A0A9E7DT30 (Residues: 1-229; Coverage: 100%)
Gene name: Pam3_19
Sequence domains: Baseplate hub gp41
Phage protein Gp138 N-terminal domain-containing protein Chains: w, x, y
Molecule details ›
Chains: w, x, y
Length: 221 amino acids
Theoretical weight: 23.54 KDa
Source organism: uncultured cyanophage
UniProt:
  • Canonical: A0A9E7DT29 (Residues: 1-221; Coverage: 100%)
Gene name: Pam3_20
Sequence domains: Phage protein Gp138 N-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.19Å
Relevant EMDB volumes: EMD-33802

Quick links

Citations

1 citations in other articles

High-resolution cryo-EM structure of the Pseudomonas bacteriophage E217.
Li et al. (2023)