› 7yib

X-ray diffraction
2.18Å resolution

Crystal structure of wild-type Cap4 SAVED domain-containing receptor from Enterobacter cloacae

Released:
DOI: 10.2210/pdb7yib/pdb
PDB entry 7yib coloured by chain, front view.
PDB entry 7yib coloured by chain, side view.
PDB entry 7yib coloured by chain, top view.
Source organism: Enterobacter cloacae
Primary publication:
Specific recognition of cyclic oligonucleotides by Cap4 for phage infection.
Chang JJ, You BJ, Tien N, Wang YC, Yang CS, Hou MH, Chen Y
Int J Biol Macromol 237 123656 (2023)
PMID: 36796558

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-535819 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CD-NTase-associated protein 4 Chain: A
Molecule details ›
Chain: A
Length: 500 amino acids
Theoretical weight: 56.6 KDa
Source organism: Enterobacter cloacae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DUD5 (Residues: 1-499; Coverage: 100%)
Gene names: P853_02261, cap4
Sequence domains: SMODS-associated and fused to various effectors sensor domain

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P21212
Unit cell:
a: 150.958Å b: 70.947Å c: 56.468Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.166 0.2
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

The arms race between bacteria CBASS and bacteriophages.
Wang et al. (2023)

2 mentions without citation

Discovery of antiphage systems in the lactococcal plasmidome.
Grafakou et al. (2024)
1 more