› 7yj4

Electron Microscopy
3.19Å resolution

Cryo-EM structure of the INSL5-bound human relaxin family peptidereceptor 4 (RXFP4)-Gi complex

Released:
DOI: 10.2210/pdb7yj4/pdb
PDB entry 7yj4 coloured by chain, front view.
PDB entry 7yj4 coloured by chain, side view.
PDB entry 7yj4 coloured by chain, top view.
Source organisms:
Primary publication:
Ligand recognition mechanism of the human relaxin family peptide receptor 4 (RXFP4).
Chen Y, Zhou Q, Wang J, Xu Y, Wang Y, Yan J, Wang Y, Zhu Q, Zhao F, Li C, Chen CW, Cai X, Bathgate RAD, Shen C, Eric Xu H, Yang D, Liu H, Wang MW
OpenAccess logo Nat Commun 14 492 (2023)
PMID: 36717591
Related structures: EMD-33871

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-225039 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
Insulin-like peptide INSL5 A chain Chain: A
Molecule details ›
Chain: A
Length: 21 amino acids
Theoretical weight: 2.22 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9Y5Q6 (Residues: 115-135; Coverage: 19%)
Gene names: INSL5, UNQ156/PRO182
Insulin-like peptide INSL5 B chain Chain: B
Molecule details ›
Chain: B
Length: 24 amino acids
Theoretical weight: 2.85 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9Y5Q6 (Residues: 23-46; Coverage: 21%)
Gene names: INSL5, UNQ156/PRO182
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63212 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Guanine nucleotide-binding protein G(i) subunit alpha-2 Chain: I
Molecule details ›
Chain: I
Length: 355 amino acids
Theoretical weight: 40.5 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P04899 (Residues: 1-355; Coverage: 100%)
Gene names: GNAI2, GNAI2B
Sequence domains: G-protein alpha subunit
Relaxin-3 receptor 2 Chain: R
Molecule details ›
Chain: R
Length: 374 amino acids
Theoretical weight: 41.18 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8TDU9 (Residues: 1-374; Coverage: 100%)
Gene names: GPCR142, GPR100, RLN3R2, RXFP4
Sequence domains: 7 transmembrane receptor (rhodopsin family)
scFv16 Chain: S
Molecule details ›
Chain: S
Length: 248 amino acids
Theoretical weight: 26.41 KDa
Source organism: synthetic construct
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: T
Molecule details ›
Chain: T
Length: 345 amino acids
Theoretical weight: 37.92 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62871 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.19Å
Relevant EMDB volumes: EMD-33871
Expression system: Spodoptera frugiperda

Quick links

Citations

1 citations in other articles

Structural insights into ligand recognition and subtype selectivity of the human melanocortin-3 and melanocortin-5 receptors.
Feng et al. (2023)