› 7ylz

X-ray diffraction
2.72Å resolution

Unliganded form of hydroxyamidotransferase TsnB9

Released:
DOI: 10.2210/pdb7ylz/pdb
PDB entry 7ylz coloured by chain, front view.
PDB entry 7ylz coloured by chain, side view.
PDB entry 7ylz coloured by chain, top view.
Source organism: Streptomyces sp. RM72
Primary publication:
Substrate Recognition Mechanism of a Trichostatin A-Forming Hydroxyamidotransferase.
Nagata R, Nishiyama M, Kuzuyama T
Biochemistry (2023)
PMID: 37167424

Function and Biology Details

Reaction catalysed: 
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-216495 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
asparagine synthase (glutamine-hydrolyzing) Chains: A, B
Molecule details ›
Chains: A, B
Length: 623 amino acids
Theoretical weight: 70.42 KDa
Source organism: Streptomyces sp. RM72
Expression system: Streptomyces albus
UniProt:
  • Canonical: A0A1Y1BFV3 (Residues: 1-611; Coverage: 100%)
Gene name: tsnB9
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 10 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P1
Unit cell:
a: 67.68Å b: 76.57Å c: 82.14Å
α: 101.153° β: 114.435° γ: 109.556°
R-values:
R R work R free
0.268 0.266 0.294
Expression system: Streptomyces albus

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