› 7ymh

Electron Microscopy
3.52Å resolution

Cryo-EM structure of Nb29-alpha1AAR-miniGsq complex bound to noradrenaline

Released:
DOI: 10.2210/pdb7ymh/pdb
PDB entry 7ymh coloured by chain, front view.
PDB entry 7ymh coloured by chain, side view.
PDB entry 7ymh coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of α1A-adrenergic receptor activation and recognition by an extracellular nanobody.
Toyoda Y, Zhu A, Kong F, Shan S, Zhao J, Wang N, Sun X, Zhang L, Yan C, Kobilka BK, Liu X
OpenAccess logo Nat Commun 14 3655 (2023)
PMID: 37339967
Related structures: EMD-33928

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-235625 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Alpha-1A adrenergic receptor; Endolysin Chain: A
Molecule details ›
Chain: A
Length: 480 amino acids
Theoretical weight: 53.74 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P35348 (Residues: 1-222, 223-227, 262-371; Coverage: 72%)
  • Canonical: D9IEF7 (Residues: 61-161; Coverage: 62%)
Gene names: ADRA1A, ADRA1C, T4Tp126, e
Sequence domains:
miniGsq Chain: B
Molecule details ›
Chain: B
Length: 246 amino acids
Theoretical weight: 28.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Nb29 Chain: D
Molecule details ›
Chain: D
Length: 144 amino acids
Theoretical weight: 15.82 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand E5E 1 x E5E
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.52Å
Relevant EMDB volumes: EMD-33928
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

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