› 7yo6

X-ray diffraction
2.54Å resolution

Bifunctional xylosidase/glucosidase LXYL with intermediate substrate xylose for 5 sec

Released:
DOI: 10.2210/pdb7yo6/pdb
PDB entry 7yo6 coloured by chain, front view.
PDB entry 7yo6 coloured by chain, side view.
PDB entry 7yo6 coloured by chain, top view.
Source organism: Lentinula edodes
Entry author: Yang LY

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124603 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Probable beta-glucosidase G Chains: A, B
Molecule details ›
Chains: A, B
Length: 803 amino acids
Theoretical weight: 86.04 KDa
Source organism: Lentinula edodes
Expression system: Escherichia coli
UniProt:
  • Canonical: G8GLP2 (Residues: 1-803; Coverage: 100%)
Gene name: Lxyl-p1-2
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, MAN
Ligand NAG 3 x NAG
Ligand MAN 1 x MAN
Carbohydrate polymer : NEW Components: NAG
Ligand NAG 10 x NAG
Carbohydrate polymer : NEW Components: NAG, MAN
Ligand NAG 2 x NAG
Ligand MAN 2 x MAN
4 bound ligands:
Ligand NAG 6 x NAG
Ligand BKR 2 x BKR
Ligand XYZ 1 x XYZ
Ligand XYL 1 x XYL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: C2221
Unit cell:
a: 79.594Å b: 181.92Å c: 241.96Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.19 0.265
Expression system: Escherichia coli

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