› 7yp7

Electron Microscopy
3.1Å resolution

apo-ADGRG2 coupled to Gs

Released:
DOI: 10.2210/pdb7yp7/pdb
PDB entry 7yp7 coloured by chain, front view.
PDB entry 7yp7 coloured by chain, side view.
PDB entry 7yp7 coloured by chain, top view.
Source organisms:
Primary publication:
apo-ADGRG2 coupled to Gs
Liu ZM, Bu RQ, Xue CY, Guo SC, Yuan DP, Xiao P, Sun JP
Nat. Chem. Biol. (2022)
Related structures: EMD-33995

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-225703 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 45.68 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 100%)
  • Best match: P63092-4 (Residues: 1-395)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 358 amino acids
Theoretical weight: 39.49 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nano-body 35 Chain: N
Molecule details ›
Chain: N
Length: 160 amino acids
Theoretical weight: 17.35 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Adhesion G-protein coupled receptor G2 Chain: R
Molecule details ›
Chain: R
Length: 903 amino acids
Theoretical weight: 98.93 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8CJ12 (Residues: 38-891; Coverage: 88%)
  • Best match: Q8CJ12-5 (Residues: 40-864)
Gene names: Adgrg2, Gpr64, Me6
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-33995
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

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