› 7yqe

X-ray diffraction
3.5Å resolution

Structure of human SRC regulatory domains in complex with the C-terminal PRRP motifs of GPR54.

Released:
DOI: 10.2210/pdb7yqe/pdb
PDB entry 7yqe coloured by chain, front view.
PDB entry 7yqe coloured by chain, side view.
PDB entry 7yqe coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Kisspeptin-10 binding to Gpr54 in osteoclasts prevents bone loss by activating Dusp18-mediated dephosphorylation of Src.
Li Z, Yang X, Fu R, Wu Z, Xu S, Jiao J, Qian M, Zhang L, Wu C, Xie T, Yao J, Wu Z, Li W, Ma G, You Y, Chen Y, Zhang HK, Cheng Y, Tang X, Wu P, Lian G, Wei H, Zhao J, Xu J, Ai L, Siwko S, Wang Y, Ding J, Song G, Luo J, Liu M, Xiao J
OpenAccess logo Nat Commun 15 1300 (2024)
PMID: 38346942

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-276148 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
KiSS-1 receptor; Proto-oncogene tyrosine-protein kinase Src Chains: A, B
Molecule details ›
Chains: A, B
Length: 188 amino acids
Theoretical weight: 21.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P12931 (Residues: 85-247; Coverage: 30%)
  • Canonical: Q969F8 (Residues: 333-357; Coverage: 6%)
Gene names: AXOR12, GPR54, KISS1R, SRC, SRC1
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P3121
Unit cell:
a: 95.245Å b: 95.245Å c: 122.82Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.27 0.268 0.295
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

Kisspeptin-10 binding to Gpr54 in osteoclasts prevents bone loss by activating Dusp18-mediated dephosphorylation of Src.
Li et al. (2024)