› 7yrp

X-ray diffraction
2Å resolution

Crystal structure of VIM-28 metallo-beta-lactamase

Released:
DOI: 10.2210/pdb7yrp/pdb
PDB entry 7yrp coloured by chain, front view.
PDB entry 7yrp coloured by chain, side view.
PDB entry 7yrp coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Entry authors: Shimizu-Ibuka A, Sato H

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-218493 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase type 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 247 amino acids
Theoretical weight: 26.12 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: F8UTU0 (Residues: 21-266; Coverage: 100%)
Gene name: blaVIM-28
Sequence domains: Metallo-beta-lactamase superfamily

Ligands and Environments

3 bound ligands:
Ligand FLC 2 x FLC
Ligand ZN 4 x ZN
Ligand GOL 6 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: C2
Unit cell:
a: 139.36Å b: 45.91Å c: 103.26Å
α: 90° β: 103.59° γ: 90°
R-values:
R R work R free
0.173 0.172 0.204
Expression system: Escherichia coli

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