› 7yu2

X-ray diffraction
1.21Å resolution

Structure of 6-aminohexanoate-oligomer hydrolase NylC, D122G/H130Y/T267C mutant, hydroxylamine-treated

Released:
DOI: 10.2210/pdb7yu2/pdb
PDB entry 7yu2 coloured by chain, front view.
PDB entry 7yu2 coloured by chain, side view.
PDB entry 7yu2 coloured by chain, top view.
Source organism: Arthrobacter
Primary publication:
X-ray crystallographic and mutational analysis of the NylC precursor: Catalytic mechanism of autocleavage and substrate hydrolysis of nylon hydrolase.
Negoro S, Shibata N, Kato DI, Tanaka Y, Yasuhira K, Nagai K, Oshima S, Furuno Y, Yokoyama R, Miyazaki K, Takeo M, Hengphasatporn K, Shigeta Y, Lee YH, Higuchi Y
FEBS J (2023)
PMID: 36799721

Function and Biology Details

Reaction catalysed: 
(N-(6-aminohexanoyl))(n) + H(2)O = (N-(6-aminohexanoyl))(n-x) + (N-(6-aminohexanoyl))(x)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181257 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
6-aminohexanoate-oligomer endohydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 355 amino acids
Theoretical weight: 36.91 KDa
Source organism: Arthrobacter
Expression system: Escherichia coli
UniProt:
  • Canonical: Q79F77 (Residues: 1-355; Coverage: 100%)
Gene name: nylC
Sequence domains: Peptidase family S58

Ligands and Environments

2 bound ligands:
Ligand GOL 8 x GOL
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: C2221
Unit cell:
a: 70.27Å b: 144.022Å c: 128.262Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.117 0.116 0.143
Expression system: Escherichia coli

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