› 7yuj

X-ray diffraction
1.86Å resolution

Crystal structure of HOIL-1L(365-510)

Released:
DOI: 10.2210/pdb7yuj/pdb
PDB entry 7yuj coloured by chain, front view.
PDB entry 7yuj coloured by chain, side view.
PDB entry 7yuj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mechanistic insights into the enzymatic activity of E3 ligase HOIL-1L and its regulation by the linear ubiquitin chain binding.
Xu X, Wang Y, Zhang Y, Wang Y, Yin Y, Peng C, Gong X, Li M, Zhang Y, Zhang M, Tang Y, Zhou X, Liu H, Pan L
OpenAccess logo Sci Adv 9 eadi4599 (2023)
PMID: 37831767

Function and Biology Details

Reaction catalysed: 
(1a) [E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [RBR-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [RBR-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-227944 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RanBP-type and C3HC4-type zinc finger-containing protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 150 amino acids
Theoretical weight: 16.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BYM8 (Residues: 365-510; Coverage: 29%)
Gene names: C20orf18, RBCK1, RNF54, UBCE7IP3, XAP3, XAP4

Ligands and Environments

2 bound ligands:
Ligand ZN 10 x ZN
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P21
Unit cell:
a: 53.252Å b: 59.87Å c: 57.818Å
α: 90° β: 90.92° γ: 90°
R-values:
R R work R free
0.155 0.153 0.187
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Catalysis of non-canonical protein ubiquitylation by the ARIH1 ubiquitin ligase.
Purser et al. (2023)