› 7yyn

Electron Microscopy
6.21Å resolution

Mammalian Dicer in the dicing state with pre-miR-15a substrate

Released:
DOI: 10.2210/pdb7yyn/pdb
PDB entry 7yyn coloured by chain, front view.
PDB entry 7yyn coloured by chain, side view.
PDB entry 7yyn coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural and functional basis of mammalian microRNA biogenesis by Dicer.
Zapletal D, Taborska E, Pasulka J, Malik R, Kubicek K, Zanova M, Much C, Sebesta M, Buccheri V, Horvat F, Jenickova I, Prochazkova M, Prochazka J, Pinkas M, Novacek J, Joseph DF, Sedlacek R, Bernecky C, O'Carroll D, Stefl R, Svoboda P
OpenAccess logo Mol Cell 82 4064-4079.e13 (2022)
PMID: 36332606
Related structures: EMD-14384

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-569871 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Endoribonuclease Dicer Chain: A
Molecule details ›
Chain: A
Length: 1765 amino acids
Theoretical weight: 200.04 KDa
Source organism: Mus musculus
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: Q8R418 (Residues: 244-245, 246-1916; Coverage: 87%)
  • Best match: Q8R418-2 (Residues: 2-1678)
Gene names: Dicer, Dicer1, Mdcr
Sequence domains:
59-nt precursor of miR-15a Chain: B
Molecule details ›
Chain: B
Length: 59 nucleotides
Theoretical weight: 19.02 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.21Å
Relevant EMDB volumes: EMD-14384
Expression system: Baculovirus expression vector pFastBac1-HM

Quick links

Citations

7 review citations

microRNAs in action: biogenesis, function and regulation.
Shang et al. (2023)
6 more

2 mentions without citation

Dicer structure and function: conserved and evolving features.
Zapletal et al. (2023)
1 more