› 7yzb

X-ray diffraction
1.47Å resolution

Crystal structure of the human FoxH1 bound to the TGTGGATT site

Released:
DOI: 10.2210/pdb7yzb/pdb
PDB entry 7yzb coloured by chain, front view.
PDB entry 7yzb coloured by chain, side view.
PDB entry 7yzb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for DNA recognition by the maternal pioneer transcription factor FoxH1.
Pluta R, Aragón E, Prescott NA, Ruiz L, Mees RA, Baginski B, Flood JR, Martin-Malpartida P, Massagué J, David Y, Macias MJ
OpenAccess logo Nat Commun 13 7279 (2022)
PMID: 36435807

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-270254 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Forkhead box protein H1 Chain: A
Molecule details ›
Chain: A
Length: 185 amino acids
Theoretical weight: 20.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75593 (Residues: 1-185; Coverage: 51%)
Gene names: FAST1, FAST2, FOXH1
Sequence domains: Forkhead domain
DNA (5'-D(*AP*GP*AP*TP*TP*GP*TP*GP*GP*AP*TP*TP*GP*CP*GP*A)-3') Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.99 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*TP*CP*GP*CP*AP*AP*TP*CP*CP*AP*CP*AP*AP*TP*CP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.8 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand K 1 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P21
Unit cell:
a: 36.118Å b: 78.029Å c: 51.879Å
α: 90° β: 100.46° γ: 90°
R-values:
R R work R free
0.164 0.162 0.19
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

TGF-β signaling in health and disease.
Massagué et al. (2023)
1 other article