› 7yzf

X-ray diffraction
2.18Å resolution

Crystal structure of the human FoxA2 bound to the TGTTTATT site (forkhead motif ATAAACA)

Released:
DOI: 10.2210/pdb7yzf/pdb
PDB entry 7yzf coloured by chain, front view.
PDB entry 7yzf coloured by chain, side view.
PDB entry 7yzf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for DNA recognition by the maternal pioneer transcription factor FoxH1.
Pluta R, Aragón E, Prescott NA, Ruiz L, Mees RA, Baginski B, Flood JR, Martin-Malpartida P, Massagué J, David Y, Macias MJ
Nat Commun 13 7279 (2022)
PMID: 36435807

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122355 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Hepatocyte nuclear factor 3-beta Chain: C
Molecule details ›
Chain: C
Length: 125 amino acids
Theoretical weight: 14.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y261 (Residues: 149-273; Coverage: 27%)
Gene names: FOXA2, HNF3B, TCF3B
Sequence domains: Forkhead domain
DNA (5'-D(P*AP*GP*AP*TP*TP*GP*TP*TP*TP*AP*TP*TP*GP*AP*GP*A)-3') Chain: A
Molecule details ›
Chain: A
Length: 16 nucleotides
Theoretical weight: 4.97 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(P*TP*CP*TP*CP*AP*AP*TP*AP*AP*AP*CP*AP*AP*TP*CP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.83 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand K 1 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: C2221
Unit cell:
a: 45.459Å b: 92.695Å c: 116.842Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.222 0.257
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

TGF-β signaling in health and disease.
Massagué et al. (2023)
1 other article