› 7z0f

X-ray diffraction
2.4Å resolution

CPAP:S-TUBULIN:IIH5 ALPHAREP COMPLEX

Released:
DOI: 10.2210/pdb7z0f/pdb
PDB entry 7z0f coloured by chain, front view.
PDB entry 7z0f coloured by chain, side view.
PDB entry 7z0f coloured by chain, top view.
Source organisms:
Primary publication:
Structural convergence for tubulin binding of CPAP and vinca domain microtubule inhibitors.
Campanacci V, Urvoas A, Ammar Khodja L, Aumont-Nicaise M, Noiray M, Lachkar S, Curmi PA, Minard P, Gigant B
OpenAccess logo Proc Natl Acad Sci U S A 119 e2120098119 (2022)
PMID: 35507869

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169166 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tubulin alpha chain Chain: A
Molecule details ›
Chain: A
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Ovis aries
Tubulin beta chain Chain: B
Molecule details ›
Chain: B
Length: 445 amino acids
Theoretical weight: 50 KDa
Source organism: Ovis aries
IIH5 ALPHAREP Chain: C
Molecule details ›
Chain: C
Length: 170 amino acids
Theoretical weight: 18.87 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Centromere protein J Chain: P
Molecule details ›
Chain: P
Length: 79 amino acids
Theoretical weight: 9.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HC77 (Residues: 319-397; Coverage: 6%)
Gene names: CENPJ, CPAP, LAP, LIP1

Ligands and Environments

4 bound ligands:
Ligand GTP 1 x GTP
Ligand MG 1 x MG
Ligand GDP 1 x GDP
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21
Unit cell:
a: 54.28Å b: 86.71Å c: 137.61Å
α: 90° β: 96.61° γ: 90°
R-values:
R R work R free
0.224 0.222 0.273
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Computational Approaches to the Rational Design of Tubulin-Targeting Agents.
Pérez-Peña et al. (2023)